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Winter Flounder Antifreeze Protein Improves the Cold Hardiness of Plant Tissues

 

ABSTRACT

Exposure of plant tissues to the winter flounder antifreeze protein (AFP) has revealed three novel properties by which plant cold hardiness may be improved. Firstly, vacuum infiltration of the protein into leaves of potato, canola (Brassica napus) and Arabidopsis thaliana resulted in a significant depression of the spontaneous freezing temperature relative to water infiltrated controls. In the case of canola, the freezing temperature was decreased by an average of 1.8 °C. These results demonstrated the ability of the AFP to function as an anti-nucleator in plant tissues. Secondly, exposure of suspension cultured cells of bromegrass to the antifreeze protein resulted in a reduction in the amount of freezable water frozen at any given temperature. This showed that the protein could act as a cryoprotectant. Thirdly, the antifreeze protein decreased the rate of ice crystal formation. These results demonstrate the feasibility of improving the cold hardiness of plants by introduction of the antifreeze protein gene.

Winter Flounder Antifreeze Protein Improves the Cold Hardiness of Plant Tissues. Available from: https://www.researchgate.net/publication/257040241_Winter_Flounder_Antifreeze_Protein_Improves_the_Cold_Hardiness_of_Plant_Tissues [accessed Aug 24, 2017].

SUMMARY

The overall objective of the project was to characterize field pea lines low in phytic acid levels at biochemical and molecular level. Accumulation of phosphorus -containing compounds in developing seeds of field pea lines normal and low in phytic acid levels were assessed. The possibility of MIPS enzyme controlling the low-phytate trait in field pea cultivars was analyzed. Finally, genomic regions associated with low-phyate trait was mapped on to field pea genome.

Topics

  • Cell Biology
  • Biotechnology
  • Biochemistry
  • Organic Chemistry
  • Agricultural Plant Science
  • Cancer Biology
  • Botany

Project Personel

Fawzy Georges

Fawzy Georges

Jene Quests Corporation

Senior Research Officer Research Gate Profile
Edward Kendall

Edward Kendall

Memorial University of Newfoundland

Discipline of Radiology Research Gate Link
Garth Fletcher

Garth Fletcher

Memorial University of Newfoundland

Department of Ocean Sciences Research Gate Profile

CONTRIBUTION

1st Adrian J. Cutler
2nd M. Saleem
3rd Edward Kendall | Memorial University of Newfoundland
4th Lawrence V. Gusta
5th Fawzy Georges | Jene Quests Corporation
6th Garth Leonardo Fletcher | Memorial University of Newfoundland